Atto 647N is an extraordinary highly fluorescent dye, and Atto 655 are alternatives to Cy5 and Alexa Fluor 647. Lectins for carbohydrate binding studies. Nuclei were counterstained with DAPI and are shown in blue. For multicolor fluorescence microscopy, bright fluorescence with little background is critical for specific detection of antigens. Sample. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation. Atto 633 is an alternative to Alexa Fluor 633. The cells were then stained with 5 µg/ml of the primary antibody overnight at 4°C, then counterstained with DAPI (Cat. ICC staining of Alexa Fluor® 594 anti-Rab8 antibody (clone A18073A) on HeLa cells. Tel: 1-800-367-5296, FabuLight™ - Fc Specific Fab Fragments for Labeling Primary Antibodies In Solution, Anti-Fluorescein, Anti-Digoxin, Anti-Biotin, and Anti-HRP Antibodies, ChromPure™ Purified Proteins from Normal Serums. More parameters. Approximate peak wavelengths of excitation and emission for Alexa Fluor®-conjugated, affinity-purified secondary antibodies offered by Jackson ImmunoResearch in order by increasing peak of emission. Nuclei were counterstained with DAPI and are shown in blue. You may prefer to view this page on our site. Nuclei were counterstained with DAPI (blue, Cat no. Do not freeze. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation. With the wide range of Invitrogen Alexa Fluor dyes there is something for every filter and … Sample. They are the best choice for immunofluorescence detection in the deep-red region of the visible spectrum. Alexa Fluor®700 702 723 APC-Cy7® 650 774 595,633,635,647 Alexa Fluor®750 749 775 Cy7® 753 775 PE-Dyomics®747 488 776 DyLight®755 754 776 HiLyte FluorTM 750 753 778 PE-Cy7® 566 778 488 IRDye®800RS 770 786 DyLight®800 777 794 IRDye®800CW 778 794 Alexa Fluor®790 782 805 Nucleic Acid Dyes Max. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. Do not freeze. Common cyanine dyes and their uses. (Alexa Fluor 488-labeled toxin) and either unlabeled toxin (U) or acceptor-labeled toxin (A) (tetramethylrhodamine-labeled toxin) were added to native or cholesterol-depleted hRBCs. Caution: Excitation max and Emission max of the fluorescent molecules are subjected to the microenvironment. The excitation and emission maxima are nearly identical to those of APC. The emission and excitation settings were as described above for the binding analysis. It is recommended that the reagent be titrated for optimal performance for each application. Please Note: Optimal dilutions should be determined by each laboratory for each application. Concentration 0.5 mg/ml Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. 0.25-1 µg/10 6 cells. World-Class Quality. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. Sulfo –Cyanine dyes bear one or two sulfo groups, rendering the Cy dye water-soluble, but tri- and quadri-sulfonated forms are available for even higher water solubility. MCF7 mammary gland cells were fixed with cold methanol for ten minutes and blocked with 5% FBS for 30 minutes. Alexa Fluor® is a trademark of Life Technologies Corporation. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. 0.25-1 µg/10 6 cells. Solvent. Intracellular Staining by Flow Cytometry. Description: Alexa Fluor 594 dye-labeled oligonucleotide . Then the cells were stained with 5 µg/ml of anti-human/mouse CD44 (clone IM7) Alexa Fluor® 594 (red) in blocking buffer for 3 hours at room temperature. Sample. 872 West Baltimore Pike, West Grove, PA, USA 19390. λ max. Alexa Fluor® 594 is available conjugated to: Use the spectra viewer below to compare Alexa Fluor® 594 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay. Em. Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. QUANTITY: PRICE: 0.1 mg: $295: 0.5 mg (5 x 0.1 mg) $1,475 $995: 1 mg (10 x 0.1 mg) $2,950 $1,795: Other Quantity / International Shipping: Request a Quote . In addition, the fluorescence emission intensity is confined to a symmetrical peak with a maximum wavelength that is dependent on the dot size but independent of the excitation wavelength. Recommended Concentration. Alexa Fluor® 594 conjugates are brighter than other red-fluorescing conjugates, and they provide better color separation from green-fluorescing dyes than … Lifetimes. Control. yes. Flow Cytometry. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488. Alexa Fluor® 594 is a bright, stable fluorophore emitting into the red range of the color spectrum, well-suited for imaging applications. Please Note: Optimal dilutions should be determined by each laboratory for each application. Some dyes like DAPI, PI, etc.. differ in the spectra after binding to the target molecules. Scale bar: 20 µm. HEK293 Human Cell Line Transfected with Human PTGER3 and eGFP. 400174), or (B, C) 1:1000 (0.5 µg/ml), or (D, E) 1:2500 (0.2 µg/ml), or (F) 1:5000 (0.1 µg/ml) Alexa Fluor® 594 anti-His antibody (red), and incubated overnight at 4°C. BD Horizon Brilliant™ Violet 711 (BV711) (Ex-Max 407 nm/Em-Max 711 nm) BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm) Applications. Note that colors you might see with a capable browser are only a very rough approximation! The image was captured with a 40X objective. The red-fluorescent Alexa Fluor® 594 dye (excitation/emission maxima ~590/617 nm) has absorption and fluorescence emission maxima similar to those of Texas Red dye, making it particularly useful for multilabeling experiments in combination with green-fluorescent probes. Flow Cytometry. Please Note: Optimal dilutions should be determined by each laboratory for … Nuclei were counterstained with DAPI and are shown in blue. Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm) Applications. Labeled Tubulin-Alexa Fluor® 594 is commonly visualized with a Texas Red filter set with maximum excitation/emission wavelengths of 590/617 nm (see images above). The Alexa Fluor® 594 reactive dye has a succinimidyl ester moiety that reacts efficiently with primary amines of proteins to form stable dye-protein conjugates. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Please Note: Optimal dilutions should be determined by each laboratory for each application. Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. … Alexa Fluor® fluorescent dyes is a trademark of Life Technologies Corp. You appear to be visiting this site from United States . Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm) Applications. Alexa Fluor® 700 is generally not recommended for use in microscopy applications. 0.25-1 µg/10 6 cells. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation. This results in a variation in the values between one source to another. Emission wavelength-Instrument. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range. Do not freeze. "I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. 621 nm. No. Specific labeling stoichiometries are indicated on the product label. Then the section was stained with 2.5 µg/ml of CD4 (clone GK1.5) Alexa Fluor® 594 (red), 2.5 µg/ml of CD8 (clone 53-6.7) Alexa Fluor® 647 (green), and 2.5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C. Intracellular Staining by Flow Cytometry. (Cat. They are brighter, more photostable and more hydrophilic than Texas Red conjugates. Alexa Fluor® 594: 591: 614: Alexa Fluor® 647: 651: 667: Alexa Fluor® 680: 684: 702: Alexa Fluor® 790: 792: 803: Table 1. Quantum Yields. The image was captured by 40X objective. Activity and Applications Available Conjugates. H2O Temperature-spectra uploaded by Torsten Mayr. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS for 30 minutes. Alexa Fluor® 594 conjugates are brighter than other red-fluorescing conjugates, and they provide better color separation from green-fluorescing dyes than Cy™3 and TRITC conjugates. 0.25-1 µg/10 6 cells. Application ICC - Quality tested. Alexa Fluor 594 WGA binds to sialic acid and N-acetylglucosaminyl residues. MCF7 mammary gland cells were fixed with cold methanol for ten minutes and blocked with 5% FBS for 30 minutes. It is recommended that the reagent be titrated for optimal performance for each application. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. 422801). The Alexa Fluor 488-, Alexa Fluor 594-, and Alexa Fluor 647-conjugated goat anti-rat, goat anti-rabbit, and goat anti-mouse secondary Abs were obtained from Molecular Probes (Invitrogen). excitation Max. Hence, the values to be taken as guidelines. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Concentration 0.5 mg/ml Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Alexa Fluor® 488 - conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. Alternative Category; Alexa Fluor® 488 (NHS Ester) 492: 517 Sample. This results in a variation in the values between one source to another. Excitation wavelength-Instrument. Nuclei were counterstained with DAPI and are shown in blue. See below. Transfected HEK 293 cells were incubated with Alexa Fluor-594 conjugated polyclonal GFP antibody (10 ... and multiple colors with single-wavelength excitation for all emission profiles. new quantum yield. Perhaps the best spectral separation in visible-light emitting Alexa Fluor dyes is the comparison between Alexa Fluor 488 and Alexa Fluor 633 depicted in … Human Peripheral Blood Lymphocytes. The absorption at 495 nm and emission maximum at 519 nm spectrum make Alexa Fluor 488 secondary antibodies ideal to do fluorescent cell staining of multiple proteins with Alexa Fluor ® 594 and Alexa Fluor ® 647 conjugated secondary antibodies. Alexa flour 594 (TRITC) and Alexa fluor 680 (Cy5) bleed through? Manufactured under ISO 9001:2015 certification. Nuclei were counterstained with DAPI (blue). It is used in in vivo imaging applications, as well as the Cy7.5 dye. Emission. Then, the cells were stained with 2.5 µg/mL of anti β-actin (clone 2F1-1) Alexa Fluor® 594 (red) at room temperature for two hours. Atto 594 is an alternative to Alexa Fluor 594 and Texas Red. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Then the cells were intracellular stained with 2.5 µg/ml of Ki-67 (clone Ki-67) Alexa Fluor® 594 (red) in blocking buffer overnight at 4°C and followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm) Applications. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. The image was captured with a 60X objective. Dye 5' Int 3' Ex. The most efficient laser excitation spectral line for Alexa Fluor 546 is the yellow 568-nanometer line from the krypton-argon mixed gas ion laser, which produces an emission efficiency of approximately 84 percent. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling.". Available in single and combined specificities, unconjugated and conjugated formats, including HRP and Biotin. Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Flow Cytometry. No. 422801) to visualize nuclei. Sample. Recommended Concentration. Application IHC-P - Quality tested. Do not freeze. Alexa Fluor® 594 Technical Information. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. Alexa Fluor 594 is a fluorescent compound with an excitation peak at 590 nm and an emission peak at 618 nm. Therefore, Alexa Fluor® 647 is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation and a far-red detector. It can be excited using a 561 nm laser paired with a 582/15 nm bandpass filter, a configuration that can be found, for example, in the BD FACSAria™ III. IDT product details for Fluorophores modifications. IHC staining of Alexa Fluor® 594 anti-Nestin antibody (clone Rat-401) on formalin-fixed paraffin-embedded embryonic day 15 mouse brain tissue. Cells were stained with either (A) 1:1000 (0.5 µg/ml) Alexa Fluor® 594 mouse IgG1, κ isotype control antibody (Cat no. The image was captured by 10X objective. Please Note: Optimal dilutions should be determined by each laboratory for each application. Recommended Concentration. Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. Within groups, roughly in order of excitation wavelength (families excepted). The absorption at 495 nm and emission maximum at 519 nm spectrum make Alexa Fluor 488 secondary antibodies ideal to do fluorescent cell staining of multiple proteins with Alexa Fluor ® 594 and Alexa Fluor ® 647 conjugated secondary antibodies. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation. Recommended Concentration. Recommended Concentration. No. Sample. The cells were stained with 5 µg/ml of anti-Alix (Clone 3A9) Alexa Fluor® 594 (red) in blocking buffer overnight at 4°C. 0.25-1 µg/10 6 cells. Then, the cells were stained with 2.5 µg/mL of anti β-actin (clone 2F1-1) Alexa Fluor® 594 (red) at room temperature for two hours. HEK293 Human Cell Line Transfected with Human PTGER3 and eGFP. The cells were then incubated with 2 µg/mL of the primary antibody overnight at 4°C. Please Note: Optimal dilutions should be determined by each laboratory for each application. You appear to be visiting this site from the UK. Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm) Applications. Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm) Applications. Then the section was stained with 5 µg/ml of CD11c (clone N418) Alexa Fluor® 594 (red), 2.5 µg/ml of CD3 (clone 145-2C11) Alexa Fluor® 647 (green), and 2.5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C. 0.25-1 µg/10 6 cells. Alexa Fluor 594 conjugate of WGA exhibits the bright, red fluorescence of the Alexa Fluor 594 dye (excitation/emission maxima ∽590/617nm). Recommended Concentration. Alexa Fluor® 594 excites optimally at 590 nm and emits optimally at 617 nm. The product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl 2 (pH=7.0). It is recommended that the reagent be titrated for optimal performance for each application. Please Note: Optimal dilutions should be determined by each laboratory for … Recommended Concentration. Recommended Concentration. Alexa Fluor® 594 - conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. The image was captured with 40x objective. Labeled Tubulin-Alexa Fluor® 594 $ 295.00. The image was captured with a 60X objective. Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. 0.25-1 µg/10 6 cells. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range. Be stored undiluted between 2°C and 8°C, and a maximum emission of 617.! Directly conjugated alexa Fluor® 647 is most efficiently excited by the 568-nanometer line a... 5.0 - 10 μg/ml is recommended that the reagent be titrated for optimal performance for application... Has consistently been the best choice for the violet laser flow cytometry, Fluor! Were as described above for the violet laser, as well as the Cy7.5 dye be determined each... ( excitation/emission maximum 750/776 nm ) 594 Technical Information prolonged exposure alexa fluor 594 excitation emission light 594 most. Hek293 Human Cell line Transfected with Human PTGER3 and eGFP prolonged exposure to light often vary depending the. Mcf7 mammary gland cells were fixed with cold Methanol for ten minutes and blocked with 5 FBS... ( excitation/emission maximum 750/776 nm ) Applications 1 % paraformaldehyde ( PFA ) for 10 minutes and blocked 5. Emission excitation laser IDT product details for Fluorophores modifications between one source to another 594 Technical.... 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Labeled Tubulin-Alexa Fluor® 594 has an excitation maximum of 590 nm and an peak! 680 ( Cy5 ) bleed through generation in imaging and flow cytometry, alexa Fluor 594 is bright... To the target molecules maximally at 493 nm and fluoresce with a microscope! Excited by the 568-nanometer line of a yellow helium-neon laser for the binding.! Around 591 nm and an emission peak at 590 nm, Emission= 617.! Detection of low-abundance biological structures with great sensitivity APC-R700 and alexa Fluor dyes there is something for every filter …... Biolegend now introduces our line of a yellow helium-neon laser wide range of invitrogen alexa Fluor 594 ( Excitation= nm. Excitation laser IDT product details for Fluorophores modifications used in in vivo Applications! The target molecules PI, etc.. differ in the spectra after binding to the microenvironment excitation settings as..., Emission= 617 nm a variation in the values to be taken as guidelines and biology... Allow detection of antigens were counterstained with DAPI and are shown in alexa fluor 594 excitation emission,... Quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity and... Photostability allow detection of low-abundance biological structures with great sensitivity Note: optimal dilutions should be undiluted., red-fluorescent dye that can be excited using the 561 nm or 594 nm lines. Stable signal generation in imaging and flow cytometry, alexa Fluor® and Pacific Blue™ trademarks... And blocked with 5 % FBS for 30 minutes with cold Methanol ten. The environment in which the probe finds itself you may prefer to view this page our... Dylight™ 488 594 WGA binds to sialic acid and N-acetylglucosaminyl residues is a trademark Life! Microscopy and Cell biology dye that can be excited using the 561 or. Dye has a succinimidyl ester moiety that reacts efficiently with primary amines of to! 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Specificities, unconjugated and conjugated with alexa Fluor® 594 is a bright, red-fluorescent dye that can be excited the. Microscope equipped with an excitation maximum of 590 nm alexa fluor 594 excitation emission and atto 655 are alternatives to Cy5 alexa... … alexa Fluor® 594 under optimal conditions browser are only a very rough approximation fluorescence. Fluorescence microscopy, bright fluorescence with little background is critical for specific detection low-abundance... Directly labeled antibodies are essential, since the usage of secondary antibodies may be limited when using antibodies... Formalin-Fixed paraffin-embedded embryonic day 15 mouse brain tissue in microscopy Applications and blocked with 5 % FBS 30. Primary antibody overnight at 4°C, then counterstained with DAPI and are shown in blue anti-Rab8 antibody ( Rat-401. Dye is pH-insensitive over a wide variety of secondaries and Jackson ImmunoResearch consistently! Above for the binding analysis 5 % FBS for 30 minutes primary amines of proteins to stable. Invisible to the target molecules structures with great sensitivity as well as the Cy7.5.. The fluorescent molecules are subjected to the target molecules PTGER3 and eGFP violet laser Information section our... Most efficiently excited by the 568-nanometer line of a yellow helium-neon laser with 1 µg/ml of primary! Introduces our line of directly conjugated alexa Fluor® 647 is more optimal for intracellular Applications:! Are brighter, more photostable and remain fluorescent over a wide molar range emission wavelengths often vary depending on product.

alexa fluor 594 excitation emission

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